Anti-HYPE antibody (400-450) {FITC} (STJ506117)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationFITC
IsotypeIgG
ImmunogenSynthetic peptide corresponding to amino acids within region 400-450 on mouse Protein adenylyltransferase FICD.
General Information
| Short Description | Rabbit polyclonal antibody anti-HYPE (400-450) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | FITC |
| Concentration | 0.5-1.5 µg/µl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500-1:5, 000ELISA: 1:50, 000IP: 1:200 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | FICD |
| Gene ID | 11153 |
| Uniprot ID | FICD_HUMAN |
| Immunogen | Synthetic peptide corresponding to amino acids within region 400-450 on mouse Protein adenylyltransferase FICD. |
| Immunogen Region | 400-450 |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Auto-AMPylated in vitro.it is unclear whether auto-AMPylation is relevant in vivo. N-glycosylated.predominantly glycosylated at Asn-275. |
| Function | Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates. |
| Protein Name | Protein Adenylyltransferase FicdAmpylator FicdDe-Ampylase FicdFic Domain-Containing ProteinHuntingtin Yeast Partner EHuntingtin-Interacting Protein 13Hip-13Huntingtin-Interacting Protein E |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Protein Adenylyltransferase Ficd antibodyAnti-Ampylator Ficd antibodyAnti-De-Ampylase Ficd antibodyAnti-Fic Domain-Containing Protein antibodyAnti-Huntingtin Yeast Partner E antibodyAnti-Huntingtin-Interacting Protein 13 antibodyAnti-Hip-13 antibodyAnti-Huntingtin-Interacting Protein E antibodyAnti-FICD antibodyAnti-HIP13 antibodyAnti-HYPE antibodyAnti-UNQ3041 antibodyAnti-PRO9857 antibody |
Information sourced from Uniprot.org