• STJ73596 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73596 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73596 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Hyaluronidase 1 antibody (Internal) (STJ73596)

SKU:
STJ73596-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Hyaluronidase 1 (Internal) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HYAL1
Gene ID: 3373
Uniprot ID: HYAL1_HUMAN
Immunogen Region: Internal
Accession Number: NP_009296.1; NP_695014.1; NP_695015.1; NP_695017.1;
Specificity: This antibody is expected to recognise isoform 1 (NP_009296.1) , isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1).
Immunogen Sequence: ENTRTKESCQAIKEY
Function May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
Protein Name Hyaluronidase-1
Hyal-1
Hyaluronoglucosaminidase-1
Lung Carcinoma Protein 1
Luca-1
Database Links Reactome: R-HSA-2024101
Reactome: R-HSA-2160916
Reactome: R-HSA-2206280
Cellular Localisation Secreted
Lysosome
Alternative Antibody Names Anti-Hyaluronidase-1 antibody
Anti-Hyal-1 antibody
Anti-Hyaluronoglucosaminidase-1 antibody
Anti-Lung Carcinoma Protein 1 antibody
Anti-Luca-1 antibody
Anti-HYAL1 antibody
Anti-LUCA1 antibody

Information sourced from Uniprot.org

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