• Immunofluorescence analysis of PC-12 cells using HYAL1 Rabbit polyclonal antibody (STJ11105720) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Hep G2 cells using HYAL1 Rabbit polyclonal antibody (STJ11105720) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HYAL1 in paraffin-embedded mouse heart using HYAL1 Rabbit polyclonal antibody (STJ11105720) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HYAL1 in paraffin-embedded rat kidney using HYAL1 Rabbit polyclonal antibody (STJ11105720) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HYAL1 in paraffin-embedded human stomach using HYAL1 Rabbit polyclonal antibody (STJ11105720) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from PC-3 cells, using HYAL1 Rabbit polyclonal antibody (STJ11105720) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HYAL1 antibody (136-435) (STJ11105720)

SKU:
STJ11105720

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Hyaluronidase-1 (136-435) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HYAL1
Gene ID: 3373
Uniprot ID: HYAL1_HUMAN
Immunogen Region: 136-435
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 136-435 of human HYAL1 (NP_149349.2).
Immunogen Sequence: RWAFNWDTKDIYRQRSRALV QAQHPDWPAPQVEAVAQDQF QGAARAWMAGTLQLGRALRP RGLWGFYGFPDCYNYDFLSP NYTGQCPSGIRAQNDQLGWL WGQSRALYPSIYMPAVLEGT GKSQMYVQHRVAEAFRVAVA AGDPNLPVLPYVQIFYDTTN HFLPLDELEHSLGESAAQGA AGVVLWVSWENTRTKESCQA IKEYMDTTLGPFILNVTSGA LLCSQALCSGHGRCVRRTS
Tissue Specificity Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.
Function May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
Protein Name Hyaluronidase-1
Hyal-1
Hyaluronoglucosaminidase-1
Lung Carcinoma Protein 1
Luca-1
Database Links Reactome: R-HSA-2024101
Reactome: R-HSA-2160916
Reactome: R-HSA-2206280
Cellular Localisation Secreted
Lysosome
Alternative Antibody Names Anti-Hyaluronidase-1 antibody
Anti-Hyal-1 antibody
Anti-Hyaluronoglucosaminidase-1 antibody
Anti-Lung Carcinoma Protein 1 antibody
Anti-Luca-1 antibody
Anti-HYAL1 antibody
Anti-LUCA1 antibody

Information sourced from Uniprot.org

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