| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Huntingtin (85 to 200aa) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:500-1:2000IF 1:500-1:2000IHC-P 1:500-1:2000IHC-F 1:500-1:2000Miniarikova J Zimmer V Martier R et al. Gene Ther. 2017 Oct. PMID: 28771234Meunier C Merienne N Jollé C et al. Glia. 2016 Jul 21. PMID: 27442486Déglon N M |
| Storage Instruction: |
For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in-20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
HTT |
| Gene ID: |
3064 |
| Uniprot ID: |
HD_HUMAN |
| Immunogen Region: |
85 to 200aa |
| Accession Number: |
ENSG00000197386 |
| Specificity: |
Reacts huntingtin protein, amino acids 85-200. The antibody recognizes wild type and mutant huntingtin. |
| Immunogen: |
Purified recombinant peptide derived from within residues 85 to 200 aa of human HTT produced in E. coli |
| Tissue Specificity | Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. |
| Post Translational Modifications | Huntingtin: Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis. Huntingtin: Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. Huntingtin: Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Huntingtin, myristoylated N-terminal fragment: Myristoylated at Gly-551, following proteolytic cleavage at Asp-550. |
| Function | Huntingtin: May play a role in microtubule-mediated transport or vesicle function. Huntingtin, myristoylated N-terminal fragment: Promotes the formation of autophagic vesicles. |
| Protein Name | HuntingtinHuntington Disease ProteinHd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment |
| Database Links | Reactome: R-HSA-9022692 |
| Cellular Localisation | Huntingtin: CytoplasmNucleusEarly EndosomeThe Mutant Huntingtin Protein Colocalizes With Akap8l In The Nuclear Matrix Of Huntington Disease NeuronsShuttles Between Cytoplasm And Nucleus In A Ran Gtpase-Independent MannerRecruits Onto Early Endosomes In A Rab5- And Hap40-Dependent FashionHuntingtinMyristoylated N-Terminal Fragment: Cytoplasmic VesicleAutophagosome |
| Alternative Antibody Names | Anti-Huntingtin antibodyAnti-Huntington Disease Protein antibodyAnti-Hd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment antibodyAnti-HTT antibodyAnti-HD antibodyAnti-IT15 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
