• Western blot detection of HtrA2/Omi in CHO-K1, MEF and 3T3 cell lysates using HtrA2/Omi mouse mAb (dilution 1:1000).Predicted band size:49kDa.Observed band size:36kDa.

Anti-HTRA2 antibody [1H8-C12-F8] (STJ99318)

SKU:
STJ99318

Current Stock:
Host: Mouse
Applications: WB
Reactivity: Mouse/Hamster
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Serine protease HTRA2, mitochondrial is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 1H8-C12-F8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTRA2
Gene ID: 27429
Uniprot ID: HTRA2_HUMAN
Specificity: This antibody detects endogenous levels of HtrA2/Omi and does not cross-react with related proteins.
Immunogen: Recombinant human HtrA2/Omi protein.
Post Translational Modifications Autoproteolytically activated.
Function Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Protein Name Serine Protease Htra2 - Mitochondrial
High Temperature Requirement Protein A2
Htra2
Omi Stress-Regulated Endoprotease
Serine Protease 25
Serine Proteinase Omi
Cellular Localisation Mitochondrion Intermembrane Space
Mitochondrion Membrane
Single-Pass Membrane Protein
Predominantly Present In The Intermembrane Space
Released Into The Cytosol Following Apoptotic Stimuli
Such As Uv Treatment
And Stimulation Of Mitochondria With Caspase-8 Truncated Bid/Tbid
Isoform 1: Endoplasmic Reticulum
Alternative Antibody Names Anti-Serine Protease Htra2 - Mitochondrial antibody
Anti-High Temperature Requirement Protein A2 antibody
Anti-Htra2 antibody
Anti-Omi Stress-Regulated Endoprotease antibody
Anti-Serine Protease 25 antibody
Anti-Serine Proteinase Omi antibody
Anti-HTRA2 antibody
Anti-OMI antibody
Anti-PRSS25 antibody

Information sourced from Uniprot.org

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