• Immunofluorescence analysis of HeLa cells using HSPB8 antibody (STJ24102). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody (STJ24102) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human gastric cancer using HSPB8 antibody (STJ24102) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using HSPB8 antibody (STJ24102) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.

Anti-HSPB8 antibody (STJ24102)

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against HSPB8.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:100
Gene ID: 26353
Gene Symbol: HSPB8
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
Background The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Tissue Specificity Predominantly expressed in skeletal muscle and heart.
Function Displays temperature-dependent chaperone activity.
Database Links Reactome: R-HSA-3371571
Cellular Localisation Cytoplasm
Translocates To Nuclear Foci During Heat Shock
Protein Name Heat Shock Protein Beta-8
Alpha-Crystallin C Chain
E2-Induced Gene 1 Protein
Protein Kinase H11
Small Stress Protein-Like Protein Hsp22
Alternative Names Anti-Heat Shock Protein Beta-8 antibody
Anti-Hspb8 antibody
Anti-Alpha-Crystallin C Chain antibody
Anti-E2-Induced Gene 1 Protein antibody
Anti-Protein Kinase H11 antibody
Anti-Small Stress Protein-Like Protein Hsp22 antibody
Anti-HSPB8 antibody
Anti-CRYAC antibody
Anti-E2IG1 antibody
Anti-HSP22 antibody
Anti-PP1629 antibody

Information sourced from uniprot.org

6 months standard