• Immunofluorescence analysis of paraffin-embedded mouse heart using HSPB8/HSP22 Rabbit polyclonal antibody (STJ24102) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat heart using HSPB8/HSP22 Rabbit polyclonal antibody (STJ24102) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HSPB8/HSP22 in paraffin-embedded rat heart using HSPB8/HSP22 Rabbit polyclonal antibody (STJ24102) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HSPB8/HSP22 Rabbit polyclonal antibody (STJ24102) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HSPB8 antibody (1-196) (STJ24102)

SKU:
STJ24102

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSPB8 (1-196) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPB8
Gene ID: 26353
Uniprot ID: HSPB8_HUMAN
Immunogen Region: 1-196
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8/HSP22 (NP_055180.1).
Immunogen Sequence: MADGQMPFSCHYPSRLRRDP FRDSPLSSRLLDDGFGMDPF PDDLTASWPDWALPRLSSAW PGTLRSGMVPRGPTATARFG VPAEGRTPPPFPGEPWKVCV NVHSFKPEELMVKTKDGYVE VSGKHEEKQQEGGIVSKNFT KKIQLPAEVDPVTVFASLSP EGLLIIEAPQVPPYSTFGES SFNNELPQDSQEVTCT
Tissue Specificity Predominantly expressed in skeletal muscle and heart.
Function Displays temperature-dependent chaperone activity.
Protein Name Heat Shock Protein Beta-8
Hspb8
Alpha-Crystallin C Chain
E2-Induced Gene 1 Protein
Protein Kinase H11
Small Stress Protein-Like Protein Hsp22
Database Links Reactome: R-HSA-3371571
Cellular Localisation Cytoplasm
Nucleus
Translocates To Nuclear Foci During Heat Shock
Alternative Antibody Names Anti-Heat Shock Protein Beta-8 antibody
Anti-Hspb8 antibody
Anti-Alpha-Crystallin C Chain antibody
Anti-E2-Induced Gene 1 Protein antibody
Anti-Protein Kinase H11 antibody
Anti-Small Stress Protein-Like Protein Hsp22 antibody
Anti-HSPB8 antibody
Anti-CRYAC antibody
Anti-E2IG1 antibody
Anti-HSP22 antibody
Anti-PP1629 antibody

Information sourced from Uniprot.org

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