• Immunohistochemistry analysis of HSPB7 in paraffin-embedded Human placenta using HSPB7 Rabbit polyclonal antibody (STJ11100404) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HSPB7 in paraffin-embedded Rat heart using HSPB7 Rabbit polyclonal antibody (STJ11100404) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HSPB7 in paraffin-embedded Mouse heart using HSPB7 Rabbit polyclonal antibody (STJ11100404) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HSPB7 Rabbit polyclonal antibody (STJ11100404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSPB7 antibody (1-170) (STJ11100404)

SKU:
STJ11100404

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSPB7 (1-170) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPB7
Gene ID: 27129
Uniprot ID: HSPB7_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human HSPB7 (NP_055239.1).
Immunogen Sequence: MSHRTSSTFRAERSFHSSSS SSSSSTSSSASRALPAQDPP MEKALSMFSDDFGSFMRPHS EPLAFPARPGGAGNIKTLGD AYEFAVDVRDFSPEDIIVTT SNNHIEVRAEKLAADGTVMN TFAHKCQLPEDVDPTSVTSA LREDGSLTIRARRHPHTEHV QQTFRTEIKI
Tissue Specificity Isoform 1 is highly expressed in adult and fetal heart, skeletal muscle, and at a much lower levels in adipose tissue and in aorta. Undetectable in other tissues. Isoform 2 and isoform 3 are poorly detected in heart.
Protein Name Heat Shock Protein Beta-7
Hspb7
Cardiovascular Heat Shock Protein
Cvhsp
Cellular Localisation Cytoplasm
Nucleus
Cajal Body
Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles
Alternative Antibody Names Anti-Heat Shock Protein Beta-7 antibody
Anti-Hspb7 antibody
Anti-Cardiovascular Heat Shock Protein antibody
Anti-Cvhsp antibody
Anti-HSPB7 antibody
Anti-CVHSP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance