• Western blot analysis of various lysates, using BiP/GRP78 rabbit polyclonal antibody (STJ24096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates, using BiP/GRP78 rabbit polyclonal antibody (STJ24096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using BiP/GRP78 antibody (STJ24096) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using BiP/GRP78 antibody (STJ24096) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using BiP/GRP78 antibody (STJ24096) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using BiP/GRP78 antibody (STJ24096) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HSPA5 antibody (550-650) (STJ24096)

SKU:
STJ24096

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BiP/GRP78 (550-650) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA5
Gene ID: 3309
Uniprot ID: BIP_HUMAN
Immunogen Region: 550-650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human BiP/GRP78 (NP_005338.1).
Immunogen Sequence: EEDKKLKERIDTRNELESYA YSLKNQIGDKEKLGGKLSSE DKETMEKAVEEKIEWLESHQ DADIEDFKAKKKELEEIVQP IISKLYGSAGPPPTGEEDTA E
Post Translational Modifications AMPylated by FICD. In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins. In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity.
Function Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation. (Microbial infection) Plays an important role in viral binding to the host cell membrane and entry for several flaviruses such as Dengue virus, Zika virus and Japanese encephalitis virus. Acts as a component of the cellular receptor for Dengue virus serotype 2/DENV-2 on human liver cells. (Microbial infection) Acts as a receptor for CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi. Acts as a receptor for R.delemar CotH3 in nasal epithelial cells, which may be an early step in rhinoorbital/cerebral mucormycosis (RCM) disease progression.
Protein Name Endoplasmic Reticulum Chaperone Bip
78 Kda Glucose-Regulated Protein
Grp-78
Binding-Immunoglobulin Protein
Bip
Heat Shock Protein 70 Family Protein 5
Hsp70 Family Protein 5
Heat Shock Protein Family A Member 5
Immunoglobulin Heavy Chain-Binding Protein
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-3371453
Reactome: R-HSA-381033
Reactome: R-HSA-381042
Reactome: R-HSA-381070
Reactome: R-HSA-381183
Reactome: R-HSA-8874177
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Lumen
Melanosome
Cytoplasm
Cell Surface
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To The Cell Surface Of Epithelial Cells In Response To High Levels Of Free Iron
Alternative Antibody Names Anti-Endoplasmic Reticulum Chaperone Bip antibody
Anti-78 Kda Glucose-Regulated Protein antibody
Anti-Grp-78 antibody
Anti-Binding-Immunoglobulin Protein antibody
Anti-Bip antibody
Anti-Heat Shock Protein 70 Family Protein 5 antibody
Anti-Hsp70 Family Protein 5 antibody
Anti-Heat Shock Protein Family A Member 5 antibody
Anti-Immunoglobulin Heavy Chain-Binding Protein antibody
Anti-HSPA5 antibody
Anti-GRP78 antibody

Information sourced from Uniprot.org

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