• Immunohistochemistry analysis of HSPA4 in paraffin-embedded mouse spinal cord using HSPA4 Rabbit monoclonal antibody (STJ11103087) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HSPA4 in paraffin-embedded rat liver using HSPA4 Rabbit monoclonal antibody (STJ11103087) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HSPA4 in paraffin-embedded human liver cancer using HSPA4 Rabbit monoclonal antibody (STJ11103087) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HSPA4 Rabbit monoclonal antibody (STJ11103087) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSPA4 antibody (700-800) [S7MR] (STJ11103087)

SKU:
STJ11103087

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HSPA4 (700-800) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA4
Gene ID: 3308
Uniprot ID: HSP74_HUMAN
Immunogen Region: 700-800
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human HSPA4 (P34932).
Immunogen Sequence: EELGKQIQQYMKIISSFKNK EDQYDHLDAADMTKVEKSTN EAMEWMNNKLNLQNKQSLTM DPVVKSKEIEAKIKELTSTC SPIISKPKPKVEPPKEEQKN A
Protein Name Heat Shock 70 Kda Protein 4
Hsp70ry
Heat Shock 70-Related Protein Apg-2
Database Links Reactome: R-HSA-3371453
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Heat Shock 70 Kda Protein 4 antibody
Anti-Hsp70ry antibody
Anti-Heat Shock 70-Related Protein Apg-2 antibody
Anti-HSPA4 antibody
Anti-APG2 antibody

Information sourced from Uniprot.org

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