• Western blot analysis of extracts of various cell lines, using HSPA14 antibody (STJ29187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HSPA14 antibody (240-509) (STJ29187)

SKU:
STJ29187

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSPA14 (240-509) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA14
Gene ID: 51182
Uniprot ID: HSP7E_HUMAN
Immunogen Region: 240-509
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-509 of human HSPA14 (NP_057383.2).
Immunogen Sequence: ASEFQRSFKHDVRGNARAMM KLTNSAEVAKHSLSTLGSAN CFLDSLYEGQDFDCNVSRAR FELLCSPLFNKCIEAIRGLL DQNGFTADDINKVVLCGGSS RIPKLQQLIKDLFPAVELLN SIPPDEVIPIGAAIEAGILI GKENLLVEDSLMIECSARDI LVKGVDESGASRFTVLFPSG TPLPARRQHTLQAPGSISSV CLELYESDGKNSAKEETKFA QVVLQDLDKKENGLRDILA
Function Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
Protein Name Heat Shock 70 Kda Protein 14
Hsp70-Like Protein 1
Heat Shock Protein Hsp60
Database Links Reactome: R-HSA-3371453
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Heat Shock 70 Kda Protein 14 antibody
Anti-Hsp70-Like Protein 1 antibody
Anti-Heat Shock Protein Hsp60 antibody
Anti-HSPA14 antibody
Anti-HSP60 antibody
Anti-HSP70L1 antibody

Information sourced from Uniprot.org

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