• Western blot analysis of extracts of U-87MG cells, using HSPA13 antibody (STJ116234) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of C6 cells using HSPA13 Polyclonal Antibody (STJ116234) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using HSPA13 Polyclonal Antibody (STJ116234) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSPA13 antibody (165-471) (STJ116234)

SKU:
STJ116234

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSPA13 (165-471) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPA13
Gene ID: 6782
Uniprot ID: HSP13_HUMAN
Immunogen Region: 165-471
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 165-471 of human HSPA13 (NP_008879.3).
Immunogen Sequence: MPVANAVISVPAEFDLKQRN STIEAANLAGLKILRVINEP TAAAMAYGLHKADVFHVLVI DLGGGTLDVSLLNKQGGMFL TRAMSGNNKLGGQDFNQRLL QYLYKQIYQTYGFVPSRKEE IHRLRQAVEMVKLNLTLHQS AQLSVLLTVEEQDRKEPHSS DTELPKDKLSSADDHRVNSG FGRGLSDKKSGESQVLFETE ISRKLFDTLNEDLFQKILVP IQQVLKEGHLEKTEIDEVV
Tissue Specificity Constitutively expressed in all tissues.
Function Has peptide-independent ATPase activity.
Protein Name Heat Shock 70 Kda Protein 13
Microsomal Stress-70 Protein Atpase Core
Stress-70 Protein Chaperone Microsome-Associated 60 Kda Protein
Database Links Reactome: R-HSA-3371453
Cellular Localisation Microsome
Endoplasmic Reticulum
Alternative Antibody Names Anti-Heat Shock 70 Kda Protein 13 antibody
Anti-Microsomal Stress-70 Protein Atpase Core antibody
Anti-Stress-70 Protein Chaperone Microsome-Associated 60 Kda Protein antibody
Anti-HSPA13 antibody
Anti-STCH antibody

Information sourced from Uniprot.org

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