• Immunofluorescence analysis of U-2 OS cells using ERAB/ERAB/HSD17B10 Polyclonal Antibody (STJ27401) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Human colon using ERAB/ERAB/HSD17B10 Rabbit polyclonal antibody (STJ27401) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ERAB/HSD17B10 Rabbit polyclonal antibody (STJ27401) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-HSD17B10 antibody (1-261) (STJ27401)

SKU:
STJ27401

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSD17B10 (1-261) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSD17B10
Gene ID: 3028
Uniprot ID: HCD2_HUMAN
Immunogen Region: 1-261
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human ERAB/ERAB/HSD17B10 (NP_004484.1).
Immunogen Sequence: MAAACRSVKGLVAVITGGAS GLGLATAERLVGQGASAVLL DLPNSGGEAQAKKLGNNCVF APADVTSEKDVQTALALAKG KFGRVDVAVNCAGIAVASKT YNLKKGQTHTLEDFQRVLDV NLMGTFNVIRLVAGEMGQNE PDQGGQRGVIINTASVAAFE GQVGQAAYSASKGGIVGMTL PIARDLAPIGIRVMTIAPGL FGTPLLTSLPEKVCNFLASQ VPFPSRLGDPAEYAHLVQA
Tissue Specificity Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD.
Function Mitochondrial dehydrogenase involved in pathways of fatty acid, branched-chain amino acid and steroid metabolism. Acts as (S)-3-hydroxyacyl-CoA dehydrogenase in mitochondrial fatty acid beta-oxidation, a major degradation pathway of fatty acids. Catalyzes the third step in the beta-oxidation cycle, namely the reversible conversion of (S)-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. Preferentially accepts straight medium- and short-chain acyl-CoA substrates with highest efficiency for (3S)-hydroxybutanoyl-CoA. Acts as 3-hydroxy-2-methylbutyryl-CoA dehydrogenase in branched-chain amino acid catabolic pathway. Catalyzes the oxidation of 3-hydroxy-2-methylbutanoyl-CoA into 2-methyl-3-oxobutanoyl-CoA, a step in isoleucine degradation pathway. Has hydroxysteroid dehydrogenase activity toward steroid hormones and bile acids. Catalyzes the oxidation of 3alpha-, 17beta-, 20beta- and 21-hydroxysteroids and 7alpha- and 7beta-hydroxy bile acids. Oxidizes allopregnanolone/brexanolone at the 3alpha-hydroxyl group, which is known to be critical for the activation of gamma-aminobutyric acid receptors (GABAARs) chloride channel. Has phospholipase C-like activity toward cardiolipin and its oxidized species. Likely oxidizes the 2'-hydroxyl in the head group of cardiolipin to form a ketone intermediate that undergoes nucleophilic attack by water and fragments into diacylglycerol, dihydroxyacetone and orthophosphate. Has higher affinity for cardiolipin with oxidized fatty acids and may degrade these species during the oxidative stress response to protect cells from apoptosis. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). Essential for structural and functional integrity of mitochondria. In addition to mitochondrial dehydrogenase activity, moonlights as a component of mitochondrial ribonuclease P, a complex that cleaves tRNA molecules in their 5'-ends. Together with TRMT10C/MRPP1, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity. The MRPP1-MRPP2 subcomplex catalyzes the formation of N(1)-methylguanine and N(1)-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs.HSD17B10/MRPP2 acting as a non-catalytic subunit. The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme. Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly.
Protein Name 3-Hydroxyacyl-Coa Dehydrogenase Type-2
17-Beta-Estradiol 17-Dehydrogenase
2-Methyl-3-Hydroxybutyryl-Coa Dehydrogenase
Mhbd
3-Alpha-(17-Beta-Hydroxysteroid Dehydrogenase
Nad(+
3-Hydroxy-2-Methylbutyryl-Coa Dehydrogenase
3-Hydroxyacyl-Coa Dehydrogenase Type Ii
3alpha(Or 20beta-Hydroxysteroid Dehydrogenase
7-Alpha-Hydroxysteroid Dehydrogenase
Endoplasmic Reticulum-Associated Amyloid Beta-Peptide-Binding Protein
Mitochondrial Ribonuclease P Protein 2
Mitochondrial Rnase P Protein 2
Short Chain Dehydrogenase/Reductase Family 5c Member 1
Short-Chain Type Dehydrogenase/Reductase Xh98g2
Type Ii Hadh
Database Links Reactome: R-HSA-6785470
Reactome: R-HSA-6787450
Reactome: R-HSA-70895
Reactome: R-HSA-8868766
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-3-Hydroxyacyl-Coa Dehydrogenase Type-2 antibody
Anti-17-Beta-Estradiol 17-Dehydrogenase antibody
Anti-2-Methyl-3-Hydroxybutyryl-Coa Dehydrogenase antibody
Anti-Mhbd antibody
Anti-3-Alpha-(17-Beta-Hydroxysteroid Dehydrogenase antibody
Anti-Nad(+ antibody
Anti-3-Hydroxy-2-Methylbutyryl-Coa Dehydrogenase antibody
Anti-3-Hydroxyacyl-Coa Dehydrogenase Type Ii antibody
Anti-3alpha(Or 20beta-Hydroxysteroid Dehydrogenase antibody
Anti-7-Alpha-Hydroxysteroid Dehydrogenase antibody
Anti-Endoplasmic Reticulum-Associated Amyloid Beta-Peptide-Binding Protein antibody
Anti-Mitochondrial Ribonuclease P Protein 2 antibody
Anti-Mitochondrial Rnase P Protein 2 antibody
Anti-Short Chain Dehydrogenase/Reductase Family 5c Member 1 antibody
Anti-Short-Chain Type Dehydrogenase/Reductase Xh98g2 antibody
Anti-Type Ii Hadh antibody
Anti-HSD17B10 antibody
Anti-ERAB antibody
Anti-HADH2 antibody
Anti-MRPP2 antibody
Anti-SCHAD antibody
Anti-SDR5C1 antibody
Anti-XH98G2 antibody

Information sourced from Uniprot.org

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