• Immunofluorescence analysis of HeLa cells using HSD17B1 Rabbit polyclonal antibody (STJ112735) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using HSD17B1 Rabbit polyclonal antibody (STJ112735) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HSD17B1 in paraffin-embedded mouse heart using HSD17B1 Rabbit polyclonal antibody (STJ112735) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HSD17B1 in paraffin-embedded human placenta using HSD17B1 Rabbit polyclonal antibody (STJ112735) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HSD17B1 Rabbit polyclonal antibody (STJ112735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HSD17B1 antibody (1-328) (STJ112735)

SKU:
STJ112735

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSD17B1 (1-328) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSD17B1
Gene ID: 3292
Uniprot ID: DHB1_HUMAN
Immunogen Region: 1-328
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human HSD17B1 (NP_000404.2).
Immunogen Sequence: MARTVVLITGCSSGIGLHLA VRLASDPSQSFKVYATLRDL KTQGRLWEAARALACPPGSL ETLQLDVRDSKSVAAARERV TEGRVDVLVCNAGLGLLGPL EALGEDAVASVLDVNVVGTV RMLQAFLPDMKRRGSGRVLV TGSVGGLMGLPFNDVYCASK FALEGLCESLAVLLLPFGVH LSLIECGPVHTAFMEKVLGS PEEVLDRTDIHTFHRFYQYL AHSKQVFREAAQNPEEVAE
Function Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2). Also has 20-alpha-HSD activity. Uses preferentially NADH.
Protein Name 17-Beta-Hydroxysteroid Dehydrogenase Type 1
17-Beta-Hsd 1
20 Alpha-Hydroxysteroid Dehydrogenase
20-Alpha-Hsd
E2dh
Estradiol 17-Beta-Dehydrogenase 1
Placental 17-Beta-Hydroxysteroid Dehydrogenase
Short Chain Dehydrogenase/Reductase Family 28c Member 1
Database Links Reactome: R-HSA-193144
Reactome: R-HSA-2453902
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-17-Beta-Hydroxysteroid Dehydrogenase Type 1 antibody
Anti-17-Beta-Hsd 1 antibody
Anti-20 Alpha-Hydroxysteroid Dehydrogenase antibody
Anti-20-Alpha-Hsd antibody
Anti-E2dh antibody
Anti-Estradiol 17-Beta-Dehydrogenase 1 antibody
Anti-Placental 17-Beta-Hydroxysteroid Dehydrogenase antibody
Anti-Short Chain Dehydrogenase/Reductase Family 28c Member 1 antibody
Anti-HSD17B1 antibody
Anti-E17KSR antibody
Anti-EDH17B1 antibody
Anti-EDH17B2 antibody
Anti-EDHB17 antibody
Anti-SDR28C1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance