• Immunofluorescence analysis of NIH-3T3 cells using KRAS+HRAS+NRAS Rabbit monoclonal antibody (STJ11101782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using KRAS+HRAS+NRAS Rabbit monoclonal antibody (STJ11101782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using KRAS+HRAS+NRAS Rabbit monoclonal antibody (STJ11101782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HRAS/KRAS/KRAS antibody (1-188) [S2MR] (STJ11101782)

SKU:
STJ11101782

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-KRAS+HRAS+NRAS (1-188) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRAS
HRAS
Gene ID: 3845
3265
Uniprot ID: RASK_HUMAN
RASH_HUMAN
Immunogen Region: 1-188
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human NRAS (P01111).
Immunogen Sequence: MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPTIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNSKSFADINLYREQI KRVKDSDDVPMVLVGNKCDL PTRTVDTKQAHELAKSYGIP FIETSAKTRQGVEDAFYTLV REIRQYRMKKLNSSDDGTQG CMGLPCVV
Tissue Specificity P01116 - NA
P01112 - Widely expressed.
Alternative Antibody Names Anti-Gtpase Kras antibody
Anti-K-Ras 2 antibody
Anti-Ki-Ras antibody
Anti-C-K-Ras antibody
Anti-C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed antibody
Anti-KRAS antibody
Anti-KRAS2 antibody
Anti-RASK2 antibody
Anti-Gtpase Hras antibody
Anti-H-Ras-1 antibody
Anti-Ha-Ras antibody
Anti-Transforming Protein P21 antibody
Anti-C-H-Ras antibody
Anti-P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed antibody
Anti-HRAS antibody
Anti-HRAS1 antibody

Information sourced from Uniprot.org

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