• Western blot analysis of extracts of various cell lines, using HPSE2 antibody (STJ110547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using HPSE2 antibody (STJ110547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate using HPSE2 antibody (STJ110547) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using HPSE2 antibody (STJ110547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HPSE2 antibody (42-242) (STJ110547)

SKU:
STJ110547

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HPSE2 (42-242) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPSE2
Gene ID: 60495
Uniprot ID: HPSE2_HUMAN
Immunogen Region: 42-242
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-242 of human HPSE2 (NP_068600.4).
Immunogen Sequence: GDRRPLPVDRAAGLKEKTLI LLDVSTKNPVRTVNENFLSL QLDPSIIHDGWLDFLSSKRL VTLARGLSPAFLRFGGKRTD FLQFQNLRNPAKSRGGPGPD YYLKNYEDDIVRSDVALDKQ KGCKIAQHPDVMLELQREKA AQMHLVLLKEQFSNTYSNLI LTARSLDKLYNFADCSGLHL IFALNALRRNPNNSWNSSSA L
Tissue Specificity Widely expressed, with the highest expression in brain, mammary gland, prostate, small intestine, testis and uterus. In the central nervous system, expressed in the spinal chord, caudate nucleus, thalamus, substantia nigra, medulla oblongata, putamen and pons. In the urinary bladder, expressed in longitudinal and circular layers of detrusor muscle. Found both in normal and cancer tissues.
Function Binds heparin and heparan sulfate with high affinity, but lacks heparanase activity. Inhibits HPSE, possibly by competing for its substrates (in vitro).
Protein Name Inactive Heparanase-2
Hpa2
Database Links Reactome: R-HSA-2024096
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Inactive Heparanase-2 antibody
Anti-Hpa2 antibody
Anti-HPSE2 antibody
Anti-HPA2 antibody

Information sourced from Uniprot.org

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