![Western blot analysis of lysates from wild type (WT) and HPCAL1 knockout (KO) HeLa cells, using [KO Validated] HPCAL1 Rabbit polyclonal antibody (STJ11100840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89774/358246/STJ11100840_1__86027.1713122581.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HPCAL1 knockout (KO) HeLa cells, using [KO Validated] HPCAL1 Rabbit polyclonal antibody (STJ11100840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and HPCAL1 knockout (KO) HeLa cells, using [KO Validated] HPCAL1 Rabbit polyclonal antibody (STJ11100840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89774/358246/STJ11100840_1__86027.1713122581.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HPCAL1 knockout (KO) HeLa cells, using [KO Validated] HPCAL1 Rabbit polyclonal antibody (STJ11100840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-HPCAL1 antibody (1-193) (STJ11100840)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HPCAL1 (1-193) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HPCAL1 |
| Gene ID | 3241 |
| Uniprot ID | HPCL1_HUMAN |
| Immunogen Region | 1-193 |
| Immunogen Sequence | MGKQNSKLRPEVLQDLRENT EFTDHELQEWYKGFLKDCPT GHLTVDEFKKIYANFFPYGD ASKFAEHVFRTFDTNGDGTI DFREFIIALSVTSRGKLEQK LKWAFSMYDLDGNGYISRSE MLEIVQAIYKMVSSVMKMPE DESTPEKRTDKIFRQMDTNN DGKLSLEEFIRGAKSDPSIV RLLQCDPSSASQF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2). |
Additional Info
| Function | May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. |
| Protein Name | Hippocalcin-Like Protein 1Calcium-Binding Protein Bdr-1Hlp2Visinin-Like Protein 3Vilip-3 |
| Database Links | |
| Cellular Localisation | MembraneLipid-Anchor |
| Alternative Antibody Names | Anti-Hippocalcin-Like Protein 1 antibodyAnti-Calcium-Binding Protein Bdr-1 antibodyAnti-Hlp2 antibodyAnti-Visinin-Like Protein 3 antibodyAnti-Vilip-3 antibodyAnti-HPCAL1 antibodyAnti-BDR1 antibody |
Information sourced from Uniprot.org