• Western blot analysis of lysates from wild type (WT) and HPCAL1 knockout (KO) HeLa cells, using [KO Validated] HPCAL1 Rabbit polyclonal antibody (STJ11100840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HPCAL1 antibody (1-193) (STJ11100840)

SKU:
STJ11100840

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HPCAL1 (1-193) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPCAL1
Gene ID: 3241
Uniprot ID: HPCL1_HUMAN
Immunogen Region: 1-193
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2).
Immunogen Sequence: MGKQNSKLRPEVLQDLRENT EFTDHELQEWYKGFLKDCPT GHLTVDEFKKIYANFFPYGD ASKFAEHVFRTFDTNGDGTI DFREFIIALSVTSRGKLEQK LKWAFSMYDLDGNGYISRSE MLEIVQAIYKMVSSVMKMPE DESTPEKRTDKIFRQMDTNN DGKLSLEEFIRGAKSDPSIV RLLQCDPSSASQF
Function May be involved in the calcium-dependent regulation of rhodopsin phosphorylation.
Protein Name Hippocalcin-Like Protein 1
Calcium-Binding Protein Bdr-1
Hlp2
Visinin-Like Protein 3
Vilip-3
Cellular Localisation Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Hippocalcin-Like Protein 1 antibody
Anti-Calcium-Binding Protein Bdr-1 antibody
Anti-Hlp2 antibody
Anti-Visinin-Like Protein 3 antibody
Anti-Vilip-3 antibody
Anti-HPCAL1 antibody
Anti-BDR1 antibody

Information sourced from Uniprot.org

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