• STJ73496 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73496 Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73496 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73496 (8µg/ml) staining of paraffin embedded Human Prostate. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ73496 Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.

Anti-HOXD13 antibody (Internal) (STJ73496)

SKU:
STJ73496-100

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Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Mouse/Rat/Cow/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-HOXD13 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-8µg/ml
IF-Strong expression of the protein seen in the nucleus of HEK293 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HOXD13
Gene ID: 3239
Uniprot ID: HXD13_HUMAN
Immunogen Region: Internal
Accession Number: NP_000514.2
Immunogen Sequence: HGVGLQQNALKSSPH
Protein Name Homeobox Protein Hox-D13
Homeobox Protein Hox-4i
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Hox-D13 antibody
Anti-Homeobox Protein Hox-4i antibody
Anti-HOXD13 antibody
Anti-HOX4I antibody

Information sourced from Uniprot.org

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