• Immunohistochemistry analysis of HOXA9 in paraffin-embedded human placenta using HOXA9 Rabbit monoclonal antibody (STJ11102989) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HOXA9 Rabbit monoclonal antibody (STJ11102989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HOXA9 antibody (173-272) [S9MR] (STJ11102989)

SKU:
STJ11102989

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HOXA9 (173-272) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HOXA9
Gene ID: 3205
Uniprot ID: HXA9_HUMAN
Immunogen Region: 173-272
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 173-272 of human HOXA9 (P31269).
Immunogen Sequence: GAFSENNAENESGGDKPPID PNNPAANWLHARSTRKKRCP YTKHQTLELEKEFLFNMYLT RDRRYEVARLLNLTERQVKI WFQNRRMKMKKINKDRAKDE
Post Translational Modifications Methylated on Arg-140 by PRMT5.methylation is critical for E-selectin induction.
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for induction of SELE/E-selectin and VCAM1 on the endothelial cells surface at sites of inflammation. Positively regulates EIF4E-mediated mRNA nuclear export and also increases the translation efficiency of ODC mRNA in the cytoplasm by competing with factors which repress EIF4E activity such as PRH.
Protein Name Homeobox Protein Hox-A9
Homeobox Protein Hox-1g
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Homeobox Protein Hox-A9 antibody
Anti-Homeobox Protein Hox-1g antibody
Anti-HOXA9 antibody
Anti-HOX1G antibody

Information sourced from Uniprot.org

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