Anti-HOMER1 antibody (Internal Region) (STJ13101196)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the internal region of human HOMER1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse, rat, monkey and xenopus.
General Information
| Short Description | Rabbit polyclonal anti-HOMER1 (Internal Region) for use in IHC and WB in Mouse, Rat and Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat/Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Homer1 |
| Gene ID | 26556 |
| Uniprot ID | HOME1_MOUSE |
| Immunogen | A synthetic peptide from the internal region of human HOMER1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse, rat, monkey and xenopus. |
| Immunogen Region | Internal Region |
| Specificity | Specific for Homer1. |
Additional Info
| Function | Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 5 counteracts the effects by competing for binding sites. Isoform 3 regulates the trafficking and surface expression of GRM5. Isoform 5 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, isoform 2 and isoform 3 to regulate synaptic metabotropic glutamate function. Isoform 5, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines. Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA. |
| Protein Name | Homer Protein Homolog 1Homer-1Vasp/Ena-Related Gene Up-Regulated During Seizure And Ltp 1Vesl-1 |
| Database Links | Reactome: R-MMU-6794361 |
| Cellular Localisation | CytoplasmPostsynaptic DensitySynapseCell ProjectionDendritic SpineIsoform 1 Inhibits Surface Expression Of Grm5 Causing It To Be Retained In The Endoplasmic ReticulumThe N-Terminal Of Isoform 2 May Facilitate Trafficking Of The Complex With Grm5 From The Endoplasmic Reticulum (Er) To The Plasma Membrane (Pm) |
| Alternative Antibody Names | Anti-Homer Protein Homolog 1 antibodyAnti-Homer-1 antibodyAnti-Vasp/Ena-Related Gene Up-Regulated During Seizure And Ltp 1 antibodyAnti-Vesl-1 antibodyAnti-Homer1 antibodyAnti-Vesl1 antibody |
Information sourced from Uniprot.org