![Western blot analysis of lysates from wild type (WT) and HNRNPR knockout (KO) 293T cells, using [KO Validated] HNRNPR Rabbit polyclonal antibody (STJ11100875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89809/358275/STJ11100875_1__14714.1713122621.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HNRNPR knockout (KO) 293T cells, using [KO Validated] HNRNPR Rabbit polyclonal antibody (STJ11100875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and HNRNPR knockout (KO) 293T cells, using [KO Validated] HNRNPR Rabbit polyclonal antibody (STJ11100875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89809/358275/STJ11100875_1__14714.1713122621.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HNRNPR knockout (KO) 293T cells, using [KO Validated] HNRNPR Rabbit polyclonal antibody (STJ11100875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-HNRNPR antibody (550-633 aa) (STJ11100875)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HNRNPR (550-633 aa) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HNRNPR |
| Gene ID | 10236 |
| Uniprot ID | HNRPR_HUMAN |
| Immunogen Region | 550-633 aa |
| Immunogen Sequence | AQQQRGRGSRGSRGNRGGNV GGKRKADGYNQPDSKRRQTN NQQNWGSQPIAQQPLQQGGD YSGNYGYNNDNQEFYQDTYG QQWK |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 550-633 of human HNRNPR (NP_005817.1). |
Additional Info
| Function | Component of ribonucleosomes, which are complexes of at least 20 other different heterogeneous nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. |
| Protein Name | Heterogeneous Nuclear Ribonucleoprotein RHnrnp R |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-72203 |
| Cellular Localisation | NucleusMicrosomeNucleoplasmCytoplasmLocalized In Cytoplasmic Mrnp Granules Containing Untranslated MrnasThe Tyrosine Phosphorylated Form Bound To Rna Is Found In Microsomes |
| Alternative Antibody Names | Anti-Heterogeneous Nuclear Ribonucleoprotein R antibodyAnti-Hnrnp R antibodyAnti-HNRNPR antibodyAnti-HNRPR antibody |
Information sourced from Uniprot.org