Anti-HNRNPK antibody (250-299 aa) (STJ93567)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human hnRNP K at the amino acid range 250-299
General Information
| Short Description | Rabbit polyclonal anti-Heterogeneous nuclear ribonucleoprotein K (250-299 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HNRNPK |
| Gene ID | 3190 |
| Uniprot ID | HNRPK_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human hnRNP K at the amino acid range 250-299 |
| Immunogen Region | 250-299 aa |
| Specificity | hnRNP K Polyclonal Antibody detects endogenous levels of hnRNP K protein. |
Additional Info
| Function | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation. |
| Protein Name | Heterogeneous Nuclear Ribonucleoprotein KHnrnp KTransformation Up-Regulated Nuclear ProteinTunp |
| Database Links | Reactome: R-HSA-4570464Reactome: R-HSA-72163Reactome: R-HSA-72203Reactome: R-HSA-9610379 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmCell ProjectionPodosomeRecruited To P53/Tp53-Responsive PromotersIn The Presence Of Functional P53/Tp53In Case Of Asfv InfectionThere Is A Shift In The Localization Which Becomes Predominantly Nuclear |
| Alternative Antibody Names | Anti-Heterogeneous Nuclear Ribonucleoprotein K antibodyAnti-Hnrnp K antibodyAnti-Transformation Up-Regulated Nuclear Protein antibodyAnti-Tunp antibodyAnti-HNRNPK antibodyAnti-HNRPK antibody |
Information sourced from Uniprot.org