• Immunofluorescence analysis of U2OS cells using HNRNPD Rabbit polyclonal antibody (STJ27679). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.
  • Immunohistochemistry analysis of HNRNPD in paraffin-embedded mouse pancreas using HNRNPD Rabbit polyclonal antibody (STJ27679) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HNRNPD in paraffin-embedded human stomach using HNRNPD Rabbit polyclonal antibody (STJ27679) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HNRNPD in paraffin-embedded rat lung using HNRNPD Rabbit polyclonal antibody (STJ27679) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HNRNPD Rabbit polyclonal antibody (STJ27679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HNRNPD antibody (1-306) (STJ27679)

SKU:
STJ27679

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNRNPD (1-306) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPD
Gene ID: 3184
Uniprot ID: HNRPD_HUMAN
Immunogen Region: 1-306
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
Immunogen Sequence: MSEEQFGGDGAAAAATAAVG GSAGEQEGAMVAATQGAAAA AGSGAGTGGGTASGGTEGGS AESEGAKIDASKNEEDEGHS NSSPRHSEAATAQREEWKMF IGGLSWDTTKKDLKDYFSKF GEVVDCTLKLDPITGRSRGF GFVLFKESESVDKVMDQKEH KLNGKVIDPKRAKAMKTKEP VKKIFVGGLSPDTPEEKIRE YFGGFGEVESIELPMDNKTN KRRGFCFITFKEEEPVKKI
Post Translational Modifications Arg-345 is dimethylated, probably to asymmetric dimethylarginine. Methylated by PRMT1, in an insulin-dependent manner. The PRMT1-mediated methylation regulates tyrosine phosphorylation.
Function Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.
Protein Name Heterogeneous Nuclear Ribonucleoprotein D0
Hnrnp D0
Au-Rich Element Rna-Binding Protein 1
Database Links Reactome: R-HSA-450408
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Cellular Localisation Nucleus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Component Of Ribonucleosomes
Cytoplasmic Localization Oscillates Diurnally
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein D0 antibody
Anti-Hnrnp D0 antibody
Anti-Au-Rich Element Rna-Binding Protein 1 antibody
Anti-HNRNPD antibody
Anti-AUF1 antibody
Anti-HNRPD antibody

Information sourced from Uniprot.org

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