• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g hnRNP A1 antibody (STJ11101361). Western blot was performed from the immunoprecipitate using hnRNP A1 antibody (STJ11101361) at a dilution of 1:1000.
  • Confocal imaging of C6 cells using hnRNP A1 Rabbit monoclonal antibody (STJ11101361, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of NIH/3T3 cells using hnRNP A1 Rabbit monoclonal antibody (STJ11101361, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using hnRNP A1 Rabbit monoclonal antibody (STJ11101361, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of hnRNP A1 in paraffin-embedded human colon using hnRNP A1 Rabbit monoclonal antibody (STJ11101361) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using hnRNP A1 Rabbit monoclonal antibody (STJ11101361) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of various lysates using hnRNP A1 Rabbit monoclonal antibody (STJ11101361) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HNRNPA1 antibody (250-350) [S1MR] (STJ11101361)

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STJ11101361

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-hnRNP A1 (250-350) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPA1
Gene ID: 3178
Uniprot ID: ROA1_HUMAN
Immunogen Region: 250-350
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human hnRNP A1 (P09651).
Immunogen Sequence: DGGYGGGGPGYSGGSRGYGS GGQGYGNQGSGYGGSGSYDS YNNGGGGGFGGGSGSNFGGG GSYNDFGNYNNQSSNFGPMK GGNFGGRSSGPYGGGGQYFA K
Post Translational Modifications Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine. Sumoylated.
Function Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1. May bind to specific miRNA hairpins. (Microbial infection) May play a role in HCV RNA replication. (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
Protein Name Heterogeneous Nuclear Ribonucleoprotein A1
Hnrnp A1
Helix-Destabilizing Protein
Single-Strand Rna-Binding Protein
Hnrnp Core Protein A1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein A1 - N-Terminally Processed
Database Links Reactome: R-HSA-6803529
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-9692914
Reactome: R-HSA-9735869
Cellular Localisation Nucleus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Shuttles Continuously Between The Nucleus And The Cytoplasm Along With Mrna
Component Of Ribonucleosomes
(Microbial Infection) In The Course Of Viral Infection
Colocalizes With Hcv Ns5b At Speckles In The Cytoplasm In A Hcv-Replication Dependent Manner
(Microbial Infection) Sars Coronavirus-2/Sars-Cov-2 Orf6 Protein Increases Accumulation To The Nucleus
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein A1 antibody
Anti-Hnrnp A1 antibody
Anti-Helix-Destabilizing Protein antibody
Anti-Single-Strand Rna-Binding Protein antibody
Anti-Hnrnp Core Protein A1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein A1 - N-Terminally Processed antibody
Anti-HNRNPA1 antibody
Anti-HNRPA1 antibody

Information sourced from Uniprot.org

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