• Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells using 1 Mu g HNMT antibody (STJ24061). Western blot was performed from the immunoprecipitate using HNMT antibody (STJ24061) at a dilution of 1:1000. ____Detection: ECL Enhanced Kit. _Exposure time: 10s.
  • Immunofluorescence analysis of U2OS cells using HNMT Rabbit polyclonal antibody (STJ24061). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using HNMT Rabbit polyclonal antibody (STJ24061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HNMT antibody (1-292) (STJ24061)

SKU:
STJ24061

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNMT (1-292) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNMT
Gene ID: 3176
Uniprot ID: HNMT_HUMAN
Immunogen Region: 1-292
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).
Immunogen Sequence: MASSMRSLFSDHGKYVESFR RFLNHSTEHQCMQEFMDKKL PGIIGRIGDTKSEIKILSIG GGAGEIDLQILSKVQAQYPG VCINNEVVEPSAEQIAKYKE LVAKTSNLENVKFAWHKETS SEYQSRMLEKKELQKWDFIH MIQMLYYVKDIPATLKFFHS LLGTNAKMLIIVVSGSSGWD KLWKKYGSRFPQDDLCQYIT SDDLTQMLDNLGLKYECYDL LSTMDISDCFIDGNENGDL
Function Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
Protein Name Histamine N-Methyltransferase
Hmt
Database Links Reactome: R-HSA-2408508
Reactome: R-HSA-70921
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Histamine N-Methyltransferase antibody
Anti-Hmt antibody
Anti-HNMT antibody

Information sourced from Uniprot.org

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