• STJ73745 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing a low level of nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-HNF4A antibody (Internal) (STJ73745)

SKU:
STJ73745-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-HNF4A (Internal) is suitable for use in ELISA and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-Recommended concentration:0.3-1µg/ml
IF-Expression of the protein seen in the nucleus of NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HNF4A
Gene ID: 3172
Uniprot ID: HNF4A_HUMAN
Immunogen Region: Internal
Accession Number: NP_849180.1; NP_000448.3; NP_787110.2; NP_001025174.1; NP_001245284.1; NP_001274111.1; NP_001274112.1
Specificity: This antibody is expected to recognise isoforms 1, 2, 4, 5, 7, 8 and 9
Immunogen Sequence: HPHLMQEHMGTN
Function Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Protein Name Hepatocyte Nuclear Factor 4-Alpha
Hnf-4-Alpha
Nuclear Receptor Subfamily 2 Group A Member 1
Transcription Factor 14
Tcf-14
Transcription Factor Hnf-4
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-383280
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 4-Alpha antibody
Anti-Hnf-4-Alpha antibody
Anti-Nuclear Receptor Subfamily 2 Group A Member 1 antibody
Anti-Transcription Factor 14 antibody
Anti-Tcf-14 antibody
Anti-Transcription Factor Hnf-4 antibody
Anti-HNF4A antibody
Anti-HNF4 antibody
Anti-NR2A1 antibody
Anti-TCF14 antibody

Information sourced from Uniprot.org

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