Anti-HNF-4-alpha antibody [SRM13083] (STJ11107813)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal HNF-4-alpha antibody for use in WB, IHC-P, ELISA and ChIP in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM13083 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:6000IHC-P, 1:200-1:2000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP, 5 Mu g antibody for 10 Mu g-15 Mu g of Chromatin |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Hnf4a |
| Gene ID | 15378 |
| Uniprot ID | HNF4A_MOUSE |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Immunogen Sequence | Mouse |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residue(s).phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold. |
| Function | Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitatating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells. |
| Protein Name | Hepatocyte Nuclear Factor 4-AlphaHnf-4-AlphaNuclear Receptor Subfamily 2 Group A Member 1Transcription Factor 14Tcf-14Transcription Factor Hnf-4 |
| Database Links | Reactome: R-MMU-383280 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Hepatocyte Nuclear Factor 4-Alpha antibodyAnti-Hnf-4-Alpha antibodyAnti-Nuclear Receptor Subfamily 2 Group A Member 1 antibodyAnti-Transcription Factor 14 antibodyAnti-Tcf-14 antibodyAnti-Transcription Factor Hnf-4 antibodyAnti-Hnf4a antibodyAnti-Hnf-4 antibodyAnti-Hnf4 antibodyAnti-Nr2a1 antibodyAnti-Tcf14 antibody |
Information sourced from Uniprot.org