Anti-HMGN1 antibody (1-100) (STJ27908)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HMGN1 (1-100) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMGN1 |
| Gene ID | 3150 |
| Uniprot ID | HMGN1_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MPKRKVSSAEGAAKEEPKRR SARLSAKPPAKVEAKPKKAA AKDKSSDKKVQTKGKRGAKG KQAEVANQETKEDLPAENGE TKTEESPASDEAGEKEAKSD |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGN1 (NP_004956.5). |
Additional Info
| Post Translational Modifications | Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation. Phosphorylation favors cytoplasmic localization. |
| Function | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2. |
| Protein Name | Non-Histone Chromosomal Protein Hmg-14High Mobility Group Nucleosome-Binding Domain-Containing Protein 1 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytoplasmic Enrichment Upon PhosphorylationThe Rna Edited Version Localizes To The Nucleus |
| Alternative Antibody Names | Anti-Non-Histone Chromosomal Protein Hmg-14 antibodyAnti-High Mobility Group Nucleosome-Binding Domain-Containing Protein 1 antibodyAnti-HMGN1 antibodyAnti-HMG14 antibody |
Information sourced from Uniprot.org