Anti-HMGCR antibody (STJA0006761)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human HMGCR
General Information
| Short Description | Rabbit polyclonal anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMGCR |
| Gene ID | 3156 |
| Uniprot ID | HMDH_HUMAN |
| Immunogen | Synthesized peptide derived from human HMGCR |
| Specificity | This antibody detects endogenous levels of HMGCR at Human, Mouse, Rat |
Additional Info
| Post Translational Modifications | N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner. Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2. The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity. |
| Function | Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs. |
| Protein Name | 3-Hydroxy-3-Methylglutaryl-Coenzyme A ReductaseHmg-Coa Reductase |
| Database Links | Reactome: R-HSA-191273Reactome: R-HSA-1989781 P04035-1Reactome: R-HSA-2426168 P04035-1Reactome: R-HSA-9619665 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinPeroxisome Membrane |
| Alternative Antibody Names | Anti-3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase antibodyAnti-Hmg-Coa Reductase antibodyAnti-HMGCR antibody |
Information sourced from Uniprot.org