• Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night
  • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-HMGA1 antibody (20-60 aa) (STJ196532)

SKU:
STJ196532

€57.50 - €282.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-High mobility group protein HMG-I/HMG-Y (20-60 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGA1
Gene ID: 3159
Uniprot ID: HMGA1_HUMAN
Immunogen Region: 20-60 aa
Specificity: This antibody detects endogenous levels of human HMG-I/HMG-Y
Immunogen: Synthesized peptide derived from the human HMG-I/HMG-Y the amino acid20-60
Function HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Protein Name High Mobility Group Protein Hmg-I/Hmg-Y
Hmg-I(Y
High Mobility Group At-Hook Protein 1
High Mobility Group Protein A1
High Mobility Group Protein R
Database Links Reactome: R-HSA-162592
Reactome: R-HSA-164843
Reactome: R-HSA-175567
Reactome: R-HSA-177539
Reactome: R-HSA-180689
Reactome: R-HSA-180910
Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-High Mobility Group Protein Hmg-I/Hmg-Y antibody
Anti-Hmg-I(Y antibody
Anti-High Mobility Group At-Hook Protein 1 antibody
Anti-High Mobility Group Protein A1 antibody
Anti-High Mobility Group Protein R antibody
Anti-HMGA1 antibody
Anti-HMGIY antibody

Information sourced from Uniprot.org

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