• Western blot analysis of lysates from wild type (WT) and HMG20B knockout (KO) 293T cells, using [KO Validated] HMG20B Rabbit polyclonal antibody (STJ11100827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HMG20B antibody (1-317) (STJ11100827)

SKU:
STJ11100827

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMG20B (1-317) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMG20B
Gene ID: 10362
Uniprot ID: HM20B_HUMAN
Immunogen Region: 1-317
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human HMG20B (NP_006330.2).
Immunogen Sequence: MSHGPKQPGAAAAPAGGKAP GQHGGFVVTVKQERGEGPRA GEKGSHEEEPVKKRGWPKGK KRKKILPNGPKAPVTGYVRF LNERREQIRTRHPDLPFPEI TKMLGAEWSKLQPTEKQRYL DEAEREKQQYMKELRAYQQS EAYKMCTEKIQEKKIKKEDS SSGLMNTLLNGHKGGDCDGF STFDVPIFTEEFLDQNKARE AELRRLRKMNVAFEEQNAVL QRHTQSMSSARERLEQELA
Tissue Specificity Ubiquitously expressed in adult tissues.
Function Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
Protein Name Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily E Member 1-Related
Smarce1-Related Protein
Brca2-Associated Factor 35
Hmg Box-Containing Protein 20b
Hmg Domain-Containing Protein 2
Hmg Domain-Containing Protein Hmgx2
Sox-Like Transcriptional Factor
Structural Dna-Binding Protein Braf35
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-9679191
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Chromosome
Localized To Condensed Chromosomes In Mitosis In Conjunction With Brca2
Alternative Antibody Names Anti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily E Member 1-Related antibody
Anti-Smarce1-Related Protein antibody
Anti-Brca2-Associated Factor 35 antibody
Anti-Hmg Box-Containing Protein 20b antibody
Anti-Hmg Domain-Containing Protein 2 antibody
Anti-Hmg Domain-Containing Protein Hmgx2 antibody
Anti-Sox-Like Transcriptional Factor antibody
Anti-Structural Dna-Binding Protein Braf35 antibody
Anti-HMG20B antibody
Anti-BRAF35 antibody
Anti-HMGX2 antibody
Anti-HMGXB2 antibody
Anti-SMARCE1R antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance