Anti-HMBS antibody (160-173) (STJ73153)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-HMBS (160-173) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 40kDa band observed in lysates of cell line K562 (calculated MW of 39.3kDa according to NP_000181.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | HMBS |
| Gene ID | 3145 |
| Uniprot ID | HEM3_HUMAN |
| Accession Number | NP_000181.2; NP_001019553.1; NP_001245137.1; NP_001245138.1 |
| Immunogen Region | 160-173 |
| Immunogen Sequence | HLEFRSIRGNLNTR |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_000181.2; NP_001019553.1; NP_001245137.1; NP_001245138.1). |
Additional Info
| Function | As part of the heme biosynthetic pathway, catalyzes the sequential polymerization of four molecules of porphobilinogen to form hydroxymethylbilane, also known as preuroporphyrinogen. Catalysis begins with the assembly of the dipyrromethane cofactor by the apoenzyme from two molecules of porphobilinogen or from preuroporphyrinogen. The covalently linked cofactor acts as a primer, around which the tetrapyrrole product is assembled. In the last step of catalysis, the product, preuroporphyrinogen, is released, leaving the cofactor bound to the holodeaminase intact. |
| Protein Name | Porphobilinogen DeaminasePbg-DHydroxymethylbilane SynthaseHmbsPre-Uroporphyrinogen Synthase |
| Database Links | Reactome: R-HSA-189451 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Porphobilinogen Deaminase antibodyAnti-Pbg-D antibodyAnti-Hydroxymethylbilane Synthase antibodyAnti-Hmbs antibodyAnti-Pre-Uroporphyrinogen Synthase antibodyAnti-HMBS antibodyAnti-PBGD antibodyAnti-UPS antibody |
Information sourced from Uniprot.org