• Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from COLO cells, using HLA-DOA Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using HLA-DO Alpha Polyclonal Antibody

Anti-HLA-DOA antibody (71-120 aa) (STJ93542)

SKU:
STJ93542

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HLA class II histocompatibility antigen, DO alpha chain (71-120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HLA-DOA
Gene ID: 3111
Uniprot ID: DOA_HUMAN
Immunogen Region: 71-120 aa
Specificity: HLA-DO Alpha Polyclonal Antibody detects endogenous levels of HLA-DO Alpha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HLA-DOA at the amino acid range 71-120
Protein Name Hla Class Ii Histocompatibility Antigen - Do Alpha Chain
Mhc Dn-Alpha
Mhc Dz Alpha
Mhc Class Ii Antigen Doa
Database Links Reactome: R-HSA-2132295
Cellular Localisation Endosome Membrane
Single-Pass Type I Membrane Protein
Lysosome Membrane
Complexes With Hla-Dm Molecule During Intracellular Transport And In Endosomal/Lysosomal Compartments
Heterotetramerization Is Necessary To Exit The Er
Alternative Antibody Names Anti-Hla Class Ii Histocompatibility Antigen - Do Alpha Chain antibody
Anti-Mhc Dn-Alpha antibody
Anti-Mhc Dz Alpha antibody
Anti-Mhc Class Ii Antigen Doa antibody
Anti-HLA-DOA antibody
Anti-HLA-DNA antibody
Anti-HLA-DZA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance