• Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239). Western blot was performed from the immunoprecipitate using HK1 (STJ11101239) at a dilution of 1:1000.
  • Confocal imaging of paraffin-embedded mouse kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of U-2 OS cells using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of HK1 in paraffin-embedded rat kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HK1 in paraffin-embedded mouse kidney using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HK1 antibody (50-150) [S9MR] (STJ11101239)

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STJ11101239

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HK1 (50-150) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HK1
Gene ID: 3098
Uniprot ID: HXK1_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hexokinase 1 (P19367).
Immunogen Sequence: GLSRDFNPTATVKMLPTFVR SIPDGSEKGDFIALDLGGSS FRILRVQVNHEKNQNVHMES EVYDTPENIVHGSGSQLFDH VAECLGDFMEKRKIKDKKLP V
Tissue Specificity Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific. Isoform 4: Testis-specific.
Function Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
Protein Name Hexokinase-1
Brain Form Hexokinase
Hexokinase Type I
Hk I
Hexokinase-A
Database Links Reactome: R-HSA-5619056
Reactome: R-HSA-70171
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
The Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)
Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-Glucosamine
Leading To Relocation To The Cytosol
Alternative Antibody Names Anti-Hexokinase-1 antibody
Anti-Brain Form Hexokinase antibody
Anti-Hexokinase Type I antibody
Anti-Hk I antibody
Anti-Hexokinase-A antibody
Anti-HK1 antibody

Information sourced from Uniprot.org

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