Anti-HK1 antibody (31-80 aa) (STJ93630)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human HXK1 at the amino acid range 31-80
General Information
| Short Description | Rabbit polyclonal anti-Hexokinase-1 (31-80 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HK1 |
| Gene ID | 3098 |
| Uniprot ID | HXK1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human HXK1 at the amino acid range 31-80 |
| Immunogen Region | 31-80 aa |
| Specificity | HXK I Polyclonal Antibody detects endogenous levels of HXK I protein. |
Additional Info
| Function | Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome. |
| Protein Name | Hexokinase-1Brain Form HexokinaseHexokinase Type IHk IHexokinase-A |
| Database Links | Reactome: R-HSA-446205Reactome: R-HSA-5619056Reactome: R-HSA-70171 |
| Cellular Localisation | Mitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmCytosolThe Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer Membrane (Probable)Dissociates From The Mitochondrial Outer Membrane Following Inhibition By N-Acetyl-D-GlucosamineLeading To Relocation To The Cytosol |
| Alternative Antibody Names | Anti-Hexokinase-1 antibodyAnti-Brain Form Hexokinase antibodyAnti-Hexokinase Type I antibodyAnti-Hk I antibodyAnti-Hexokinase-A antibodyAnti-HK1 antibody |
Information sourced from Uniprot.org