• 1) Input: Hela Cell Lysate , 2) IP product: IP dilute 1:200
  • Immunofluorescence analysis of Rat-liver tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of Hela, diluted at 1) 1:2000 , 2) 1:5000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of Human-appendix tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H3 (Tri Methyl Lys79) monoclonal antibody (3G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Hela cell. 1, Bek Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Histone H3 (Tri methyl K79) antibody (STJ96993)

SKU:
STJ96993
Current Stock:
Host: Mouse
Applications: WB, IHC-P, IF, IP
Reactivity: Human, Mouse, Rat
Short Description: Mouse monoclonal primary antibody against Histone H3 (Tri methyl K79).
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 3G3
Conjugation: Unconjugated
Formulation: PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/mL
Isotype: IgG1
Dilution Range: WB 1:500-2000
IP 1:200
IF 1:200
IHC 1:50-300
Specificity: The antibody detects endogenous Histone H3 (tri methyl K79) protein.
Immunogen: Synthetic Peptide
6 months standard