Anti-Histone H2A antibody (1-100) [S4MR] (STJ11102114)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Histone H2A.Z (1-100) antibody for use in WB, ELISA and ChIP in human, mouse, rat and other samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Other |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP:5 Mu g antibody for 5 Mu g-10 Mu g of Chromatin |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | H2AZ1 |
| Gene ID | 3015 |
| Uniprot ID | H2AZ_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MAGGKAGKDSGKAKTKAVSR SQRAGLQFPVGRIHRHLKSR TTSHGRVGATAAVYSAAILE YLTAEVLELAGNASKDLKVK RITPRHLQLAIRGDEELDSL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A.Z (P0C0S5). |
Additional Info
| Post Translational Modifications | Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A.KAT2A is recruited by the XPC complex in absence of DNA damage. Acetylated on Lys-5, Lys-8 and Lys-12 during interphase.acetylation disappears at mitosis. Acetylation by the NuA4 histone acetyltransferase complex is required for hematopoietic stem cell maintenance. Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site. Not phosphorylated. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. |
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. |
| Protein Name | Histone H2a.zH2a/Z |
| Database Links | Reactome: R-HSA-977225 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone H2a.z antibodyAnti-H2a/Z antibodyAnti-H2AZ1 antibodyAnti-H2AFZ antibodyAnti-H2AZ antibody |
Information sourced from Uniprot.org