• Western blot analysis of extracts of various cell lines, using Histone H2A. Z rabbit monoclonal antibody (STJ11102114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts from wild type (WT) and Histone H2A. Z knockout (KO) 293T (KO) cells, using Histone H2A. Z antibody (STJ11102114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A. Z rabbit monoclonal antibody antibody (STJ11102114) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H2A antibody (1-100) [S4MR] (STJ11102114)

SKU:
STJ11102114

€183.13 - €799.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Histone H2A.Z (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2AZ1
Gene ID: 3015
Uniprot ID: H2AZ_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A.Z (P0C0S5).
Immunogen Sequence: MAGGKAGKDSGKAKTKAVSR SQRAGLQFPVGRIHRHLKSR TTSHGRVGATAAVYSAAILE YLTAEVLELAGNASKDLKVK RITPRHLQLAIRGDEELDSL
Post Translational Modifications Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression. Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A.KAT2A is recruited by the XPC complex in absence of DNA damage. Acetylated on Lys-5, Lys-8 and Lys-12 during interphase.acetylation disappears at mitosis. Acetylation by the NuA4 histone acetyltransferase complex is required for hematopoietic stem cell maintenance. Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site. Not phosphorylated. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Protein Name Histone H2a.z
H2a/Z
Database Links Reactome: R-HSA-977225
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H2a.z antibody
Anti-H2a/Z antibody
Anti-H2AZ1 antibody
Anti-H2AFZ antibody
Anti-H2AZ antibody

Information sourced from Uniprot.org

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