• Immunohistochemistry analysis of Histone H1t in paraffin-embedded rat lung using Histone H1t Rabbit polyclonal antibody (STJ11100449) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H1t in paraffin-embedded mouse kidney using Histone H1t Rabbit polyclonal antibody (STJ11100449) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Histone H1t in paraffin-embedded human esophageal using Histone H1t Rabbit polyclonal antibody (STJ11100449) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Histone H1t Rabbit polyclonal antibody (STJ11100449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Histone H1 antibody (150-207) (STJ11100449)

SKU:
STJ11100449

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histone H1t (150-207) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-6
Gene ID: 3010
Uniprot ID: H1T_HUMAN
Immunogen Region: 150-207
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-207 of human Histone H1t (NP_005314.2).
Immunogen Sequence: NKRAKKPRATTPKTVRSGRK AKGAKGKQQQKSPVKARASK SKLTQHHEVNVRKATSKK
Tissue Specificity Testis-specific.
Post Translational Modifications Phosphorylated in early spermatids. Citrullination at Arg-58 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Function Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration (Probable).
Protein Name Histone H1t
Testicular H1 Histone
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H1t antibody
Anti-Testicular H1 Histone antibody
Anti-H1-6 antibody
Anti-H1FT antibody
Anti-H1T antibody
Anti-HIST1H1T antibody

Information sourced from Uniprot.org

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