Western blot analysis of various lysates, using His-Tag mAb (STJ11103695) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-His-Tag antibody [S5MM] (STJ11103695)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
STJ11103695
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General Information

Short DescriptionMouse monoclonal His-Tag antibody for use in WB, IF, ICC, IP and ELISA in species independent samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/IP/ELISA
HostMouse
ReactivitySpecies Independent
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5MM
IsotypeIgG1
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:5000-1:20000
IF/ICC:1:50-1:200
IP:0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Immunogen SequenceHHHHHH
SpecificityA synthetic peptide corresponding to His tag.

Additional Info

Background Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Information sourced from Uniprot.org

Citations

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