• Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated mouse anti His-Tag antibody (STJ11103704) at 1:20000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein, using HRP-conjugated Mouse anti His-Tag monoclonal antibody (STJ11103704) at 1:20000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-His-Tag antibody [S4MM] {HRP} (STJ11103704)

SKU:
STJ11103704

Shipping:
Free Shipping
Current Stock:
Host: Mouse
Applications: WB
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-His-Tag is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MM
Conjugation: HRP
Isotype: IgG2Bk
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to His tag.
Immunogen Sequence: HHHHHH
Background This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance