• Immunohistochemistry of paraffin-embedded human colon carcinoma using HIRA antibody (STJ110759) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human lung cancer using HIRA antibody (STJ110759) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts from normal (control) and HIRA Rabbit pAb knockout (KO) 293T cells, using HIRA Rabbit pAb antibody (STJ110759) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using HIRA antibody (STJ110759) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using HIRA antibody (STJ110759) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Anti-HIRA antibody (STJ110759)

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STJ110759
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Host: Rabbit
Applications: WB, IHC
Reactivity: Human
Short Description: Rabbit polyclonal primary antibody against HIRA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:100-1:200
Uniprot ID: HIRA_HUMAN
Gene ID: 7290
Gene Symbol: HIRA
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).
Background This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Tissue Specificity Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
Function Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Database Links Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Pml Body
Primarily
Though Not Exclusively
Localized To The Nucleus
Localizes To Pml Bodies Immediately Prior To Onset Of Senescence
Protein Name Protein Hira
Tup1-Like Enhancer Of Split Protein 1
Alternative Names Anti-Protein Hira antibody
Anti-Tup1-Like Enhancer Of Split Protein 1 antibody
Anti-HIRA antibody
Anti-DGCR1 antibody
Anti-HIR antibody
Anti-TUPLE1 antibody

Information sourced from uniprot.org

6 months standard