| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Mouse/Dog/Pig/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-HIP2 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-Recommended for use at 1-3 µg/mlIHC-Recommended concentration, 1-2µg/mlELISA-antibody detection limit dilution 1:2000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
UBE2K |
| Gene ID: |
3093 |
| Uniprot ID: |
UBE2K_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_005330.1; NP_001104582.1; NP_001104583.1 |
| Specificity: |
This antibody is expected to recognise isoform 1 (NP_005330.1) , isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1). |
| Immunogen Sequence: |
SWDVETATELLLSN |
| Post Translational Modifications | Sumoylation at Lys-14 impairs catalytic activity. |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B.in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 KE2 Ubiquitin-Conjugating Enzyme KHuntingtin-Interacting Protein 2Hip-2Ubiquitin Carrier ProteinUbiquitin-Conjugating Enzyme E2-25 KdaUbiquitin-Conjugating Enzyme E2(25kUbiquitin-Conjugating Enzyme E2-25kUbiquitin-Protein Ligase |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-936440Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 K antibodyAnti-E2 Ubiquitin-Conjugating Enzyme K antibodyAnti-Huntingtin-Interacting Protein 2 antibodyAnti-Hip-2 antibodyAnti-Ubiquitin Carrier Protein antibodyAnti-Ubiquitin-Conjugating Enzyme E2-25 Kda antibodyAnti-Ubiquitin-Conjugating Enzyme E2(25k antibodyAnti-Ubiquitin-Conjugating Enzyme E2-25k antibodyAnti-Ubiquitin-Protein Ligase antibodyAnti-UBE2K antibodyAnti-HIP2 antibodyAnti-LIG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
