• Immunofluorescence analysis of U-2 OS cells using HIGD1A Rabbit polyclonal antibody (STJ116792) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HIGD1A in paraffin-embedded human colon carcinoma using HIGD1A Rabbit polyclonal antibody (STJ116792) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HIGD1A Rabbit polyclonal antibody (STJ116792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HIGD1A antibody (1-93) (STJ116792)

SKU:
STJ116792

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HIGD1A (1-93) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIGD1A
Gene ID: 25994
Uniprot ID: HIG1A_HUMAN
Immunogen Region: 1-93
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-93 of human HIGD1A (NP_054775.2).
Immunogen Sequence: MSTDTGVSLPSYEEDQGSKL IRKAKEAPFVPVGIAGFAAI VAYGLYKLKSRGNTKMSIHL IHMRVAAQGFVVGAMTVGMG YSMYREFWAKPKP
Function Proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes.
Protein Name Hig1 Domain Family Member 1a - Mitochondrial
Hypoxia-Inducible Gene 1 Protein
Rcf1 Homolog A
Rcf1a
Database Links Reactome: R-HSA-1234158
Cellular Localisation Mitochondrion Membrane
Multi-Pass Membrane Protein
Mitochondrion Inner Membrane
Alternative Antibody Names Anti-Hig1 Domain Family Member 1a - Mitochondrial antibody
Anti-Hypoxia-Inducible Gene 1 Protein antibody
Anti-Rcf1 Homolog A antibody
Anti-Rcf1a antibody
Anti-HIGD1A antibody
Anti-HIG1 antibody
Anti-HSPC010 antibody

Information sourced from Uniprot.org

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