• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g HIF1AN/FIH1 antibody (STJ27419). Western blot was performed from the immunoprecipitate using HIF1AN/FIH1 antibody (STJ27419) at a dilution of 1:1000.
  • Immunofluorescence analysis of L929 cells using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Rat kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Human breast cancer tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of HIF1AN/FIH1 in paraffin-embedded Mouse kidney tissue using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH1 Rabbit polyclonal antibody (STJ27419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HIF1AN antibody (1-349) (STJ27419)

SKU:
STJ27419

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HIF1AN/FIH1 (1-349) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HIF1AN
Gene ID: 55662
Uniprot ID: HIF1N_HUMAN
Immunogen Region: 1-349
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human HIF1AN/FIH1 (NP_060372.2).
Immunogen Sequence: MAATAAEAVASGSGEPREEA GALGPAWDESQLRSYSFPTR PIPRLSQSDPRAEELIENEE PVVLTDTNLVYPALKWDLEY LQENIGNGDFSVYSASTHKF LYYDEKKMANFQNFKPRSNR EEMKFHEFVEKLQDIQQRGG EERLYLQQTLNDTVGRKIVM DFLGFNWNWINKQQGKRGWG QLTSNLLLIGMEGNVTPAHY DEQQNFFAQIKGYKRCILFP PDQFECLYPYPVHHPCDRQ
Function Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Protein Name Hypoxia-Inducible Factor 1-Alpha Inhibitor
Factor Inhibiting Hif-1
Fih-1
Hypoxia-Inducible Factor Asparagine Hydroxylase
Database Links Reactome: R-HSA-1234174
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
Mainly Cytoplasmic Localization
But Interaction With Notch1 Results In Nuclear Localization And Interaction With Abpa3 Results In Perinuclear Localization In Macrophages
Alternative Antibody Names Anti-Hypoxia-Inducible Factor 1-Alpha Inhibitor antibody
Anti-Factor Inhibiting Hif-1 antibody
Anti-Fih-1 antibody
Anti-Hypoxia-Inducible Factor Asparagine Hydroxylase antibody
Anti-HIF1AN antibody
Anti-FIH1 antibody

Information sourced from Uniprot.org

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