Anti-HIF1AN antibody (1-100) [S3MR] (STJ11101933)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal HIF1AN/FIH1 (1-100) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S3MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HIF1AN |
| Gene ID | 55662 |
| Uniprot ID | HIF1N_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MAATAAEAVASGSGEPREEA GALGPAWDESQLRSYSFPTR PIPRLSQSDPRAEELIENEE PVVLTDTNLVYPALKWDLEY LQENIGNGDFSVYSASTHKF |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HIF1AN/FIH1 (Q9NWT6). |
Additional Info
| Function | Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. |
| Protein Name | Hypoxia-Inducible Factor 1-Alpha InhibitorFactor Inhibiting Hif-1Fih-1Hypoxia-Inducible Factor Asparagine Hydroxylase |
| Database Links | Reactome: R-HSA-1234174 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMainly Cytoplasmic LocalizationBut Interaction With Notch1 Results In Nuclear Localization And Interaction With Abpa3 Results In Perinuclear Localization In Macrophages |
| Alternative Antibody Names | Anti-Hypoxia-Inducible Factor 1-Alpha Inhibitor antibodyAnti-Factor Inhibiting Hif-1 antibodyAnti-Fih-1 antibodyAnti-Hypoxia-Inducible Factor Asparagine Hydroxylase antibodyAnti-HIF1AN antibodyAnti-FIH1 antibody |
Information sourced from Uniprot.org