Anti-HIC1 antibody (193-204) (STJ72862)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-HIC1 (193-204) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 75kDa band observed in Mouse Testis lysates (calculated MW of 76.8kDa according to Mouse NP_001091673.1 ). Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at roo |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | HIC1 |
| Gene ID | 3090 |
| Uniprot ID | HIC1_HUMAN |
| Accession Number | NP_006488.2; NP_001091672.1 |
| Immunogen Region | 193-204 |
| Immunogen Sequence | NTHCAELYASGP |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1). |
Additional Info
| Post Translational Modifications | Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1. Sumoylated on Lys-333 by a PIAS family member, which enhances interaction with MTA1, positively regulates transcriptional repression activity and is enhanced by HDAC4. |
| Function | Transcriptional repressor. Recognizes and binds to the consensus sequence '5-CGNGCGGGGCACACC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription of ACKR3, FGFBP1 and EFNA1. |
| Protein Name | Hypermethylated In Cancer 1 ProteinHic-1Zinc Finger And Btb Domain-Containing Protein 29 |
| Database Links | Reactome: R-HSA-3232118 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Hypermethylated In Cancer 1 Protein antibodyAnti-Hic-1 antibodyAnti-Zinc Finger And Btb Domain-Containing Protein 29 antibodyAnti-HIC1 antibodyAnti-ZBTB29 antibody |
Information sourced from Uniprot.org