Western blot analysis of extracts of HepG2 cells, using Hex Rabbit monoclonal antibody (STJ11103082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Hex antibody (50-150) [S2MR] (STJ11103082)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103082
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit monoclonal Hex (50-150) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS2MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolHHEX
Gene ID3087
Uniprot IDHHEX_HUMAN
Immunogen Region50-150
Immunogen SequenceTLPSPNSSFTSLVSPYRTPV YEPTPIHPAFSHHSAAALAA AYGPGGFGGPLYPFPRTVND YTHALLRHDPLGKPLLWSPF LQRPLHKRKGGQVRFSNDQT I
SpecificityA synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hex (Q03014).

Additional Info

Tissue Specificity Liver and promyelocytic leukemia cell line HL-60.
Function Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. Activator of WNT-mediated transcription in conjunction with CTNNB1. Establishes anterior identity at two levels.acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. Inhibits EIF4E-mediated mRNA nuclear export. May play a role in hematopoietic differentiation.
Protein Name Hematopoietically-Expressed Homeobox Protein Hhex
Homeobox Protein Hex
Homeobox Protein Prh
Proline-Rich Homeodomain Protein
Database Links Reactome: R-HSA-9925561
Cellular Localisation Nucleus
Nuclear Body
Cytoplasm
Alternative Antibody Names Anti-Hematopoietically-Expressed Homeobox Protein Hhex antibody
Anti-Homeobox Protein Hex antibody
Anti-Homeobox Protein Prh antibody
Anti-Proline-Rich Homeodomain Protein antibody
Anti-HHEX antibody
Anti-HEX antibody
Anti-PRH antibody
Anti-PRHX antibody

Information sourced from Uniprot.org

Citations

Product Review

!