• Western blot analysis of extracts of various cell lines, using HES5 rabbit monoclonal antibody (STJ11102544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using HES5 Rabbit monoclonal antibody (STJ11102544) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using HES5 rabbit monoclonal antibody (STJ11102544) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using HES5 Rabbit monoclonal antibody (STJ11102544) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung cancer using HES5 rabbit monoclonal antibody (STJ11102544) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using HES5 Rabbit monoclonal antibody (STJ11102544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HES5 antibody (12-142) [S4MR] (STJ11102544)

SKU:
STJ11102544

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HES5 (12-142) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HES5
Gene ID: 388585
Uniprot ID: HES5_HUMAN
Immunogen Region: 12-142
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 12-142 of human HES5 (Q5TA89).
Immunogen Sequence: SPKEKNRLRKPVVEKMRRDR INSSIEQLKLLLEQEFARHQ PNSKLEKADILEMAVSYLKH SKAFVAAAGPKSLHQDYSEG YSWCLQEAVQFLTLHAASDT QMKLLYHFQRPPAAPAAPAK EPKAPGAAPPP
Tissue Specificity Expressed in fetal heart and brain tumors.
Function Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.
Protein Name Transcription Factor Hes-5
Class B Basic Helix-Loop-Helix Protein 38
Bhlhb38
Hairy And Enhancer Of Split 5
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2197563
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Hes-5 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 38 antibody
Anti-Bhlhb38 antibody
Anti-Hairy And Enhancer Of Split 5 antibody
Anti-HES5 antibody
Anti-BHLHB38 antibody

Information sourced from Uniprot.org

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