Anti-HES1 antibody (181-280) [S2MR] (STJ11101262)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Hes1 (181-280) antibody for use in WB, IHC-P, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:1000-1:4000IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requireme |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HES1 |
| Gene ID | 3280 |
| Uniprot ID | HES1_HUMAN |
| Immunogen Region | 181-280 |
| Immunogen Sequence | FAPPPPLVPIPGGAAPPPGG APCKLGSQAGEAAKVFGGFQ VVPAPDGQFAFLIPNGAFAH SGPVIPVYTSNSGTSVGPNA VSPSSGPSLTADSMWRPWRN |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 181-280 of human HES1 (Q14469). |
Additional Info
| Post Translational Modifications | (Microbial infection) Ubiquitinated via human cytomegalovirus/HCMV protein IE1 that assembles a HES1 ubiquitination complex.leading to HES1 proteasomal degradation. |
| Function | Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage. |
| Protein Name | Transcription Factor Hes-1Class B Basic Helix-Loop-Helix Protein 39Bhlhb39Hairy And Enhancer Of Split 1Hairy HomologHairy-Like ProteinHhl |
| Database Links | Reactome: R-HSA-210744Reactome: R-HSA-2122947Reactome: R-HSA-2197563Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-8940973Reactome: R-HSA-8941856Reactome: R-HSA-9013508Reactome: R-HSA-9013695 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Hes-1 antibodyAnti-Class B Basic Helix-Loop-Helix Protein 39 antibodyAnti-Bhlhb39 antibodyAnti-Hairy And Enhancer Of Split 1 antibodyAnti-Hairy Homolog antibodyAnti-Hairy-Like Protein antibodyAnti-Hhl antibodyAnti-HES1 antibodyAnti-BHLHB39 antibodyAnti-HL antibodyAnti-HRY antibody |
Information sourced from Uniprot.org