• Immunoprecipitation analysis of 600 Mu g extracts of Rat testis cells using 3 Mu g HES1 antibody (STJ11101262). Western blot was performed from the immunoprecipitate using HES1 antibody (STJ11101262) at a dilution of 1:500.
  • Immunohistochemistry analysis of HES1 in paraffin-embedded mouse spinal cord using HES1 Rabbit monoclonal antibody (STJ11101262) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HES1 in paraffin-embedded rat brain using HES1 Rabbit monoclonal antibody (STJ11101262) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HES1 Rabbit monoclonal antibody (STJ11101262) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-HES1 antibody (181-280) [S2MR] (STJ11101262)

SKU:
STJ11101262

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hes1 (181-280) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HES1
Gene ID: 3280
Uniprot ID: HES1_HUMAN
Immunogen Region: 181-280
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 181-280 of human HES1 (Q14469).
Immunogen Sequence: FAPPPPLVPIPGGAAPPPGG APCKLGSQAGEAAKVFGGFQ VVPAPDGQFAFLIPNGAFAH SGPVIPVYTSNSGTSVGPNA VSPSSGPSLTADSMWRPWRN
Post Translational Modifications (Microbial infection) Ubiquitinated via human cytomegalovirus/HCMV protein IE1 that assembles a HES1 ubiquitination complex.leading to HES1 proteasomal degradation.
Function Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.
Protein Name Transcription Factor Hes-1
Class B Basic Helix-Loop-Helix Protein 39
Bhlhb39
Hairy And Enhancer Of Split 1
Hairy Homolog
Hairy-Like Protein
Hhl
Database Links Reactome: R-HSA-210744
Reactome: R-HSA-2122947
Reactome: R-HSA-2197563
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-8940973
Reactome: R-HSA-8941856
Reactome: R-HSA-9013508
Reactome: R-HSA-9013695
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Hes-1 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 39 antibody
Anti-Bhlhb39 antibody
Anti-Hairy And Enhancer Of Split 1 antibody
Anti-Hairy Homolog antibody
Anti-Hairy-Like Protein antibody
Anti-Hhl antibody
Anti-HES1 antibody
Anti-BHLHB39 antibody
Anti-HL antibody
Anti-HRY antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance