![Western blot analysis of lysates from wild type (WT) and HDAC8 knockout (KO) 293T cells, using [KO Validated] HDAC8 Rabbit mAb (STJ11102387) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91304/406047/STJ11102387_3__41164.1744891838.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HDAC8 knockout (KO) 293T cells, using [KO Validated] HDAC8 Rabbit mAb (STJ11102387) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Confocal imaging of HeLa cells using [KO Validated] HDAC8 Rabbit mAb (STJ11102387, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91304/406049/STJ11102387_5__04471.1744891839.jpg?c=1 "Confocal imaging of HeLa cells using [KO Validated] HDAC8 Rabbit mAb (STJ11102387, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse mAb (STJ11107556, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
Anti-HDAC8 antibody (1-100) [S7MR] (STJ11102387)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal HDAC8 (1-100) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:4000IHC-P:1:100-1:1000IF/ICC:1:100-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your s |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HDAC8 |
| Gene ID | 55869 |
| Uniprot ID | HDAC8_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MEEPEEPADSGQSLVPVYIY SPEYVSMCDSLAKIPKRASM VHSLIEAYALHKQMRIVKPK VASMEEMATFHTDAYLQHLQ KVSQEGDDDHPDSIEYGLGY |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC8 (Q9BY41). |
Additional Info
| Tissue Specificity | Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. |
| Post Translational Modifications | Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. |
| Function | Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones. |
| Protein Name | Histone Deacetylase 8Hd8Protein Deacetylase Hdac8Protein Decrotonylase Hdac8 |
| Database Links | Reactome: R-HSA-2122947Reactome: R-HSA-2467813 Q9BY41-1Reactome: R-HSA-2500257 Q9BY41-1Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-3214815Reactome: R-HSA-350054 |
| Cellular Localisation | NucleusChromosomeCytoplasmExcluded From The NucleoliFound In The Cytoplasm Of Cells Showing Smooth Muscle Differentiation |
| Alternative Antibody Names | Anti-Histone Deacetylase 8 antibodyAnti-Hd8 antibodyAnti-Protein Deacetylase Hdac8 antibodyAnti-Protein Decrotonylase Hdac8 antibodyAnti-HDAC8 antibodyAnti-HDACL1 antibodyAnti-CDA07 antibody |
Information sourced from Uniprot.org